CHO Cells Catalogue number: 603479

Chinese Hamster Ovary (CHO) Cells: A Cornerstone in Biopharmaceutical Manufacturing

Chinese hamster ovary (CHO) cells are a pivotal tool in biotechnology, widely recognized for their essential role in the development and production of biopharmaceuticals. From monoclonal antibodies and recombinant protein expression to vaccines, CHO cells have become the preferred animal cell line for biomanufacturing due to their versatility, reliability, and robust performance.

Key Advantages of CHO Cells
Proven Track Record
CHO cells have a long history of success in genetic, molecular biology, and biomanufacturing applications. Nearly 50 biotherapeutics developed using CHO cells have been approved in the USA and EU, underscoring their reliability and efficacy in antibody and protein production.

Biosafety and Stability
CHO cells’ hamster origin reduces susceptibility to human viruses, enhancing biosafety in production environments.
Their consistent performance minimizes batch-to-batch variation, a critical factor for large-scale biomanufacturing.

Post-Translational Modifications
CHO cells excel in producing therapeutic proteins requiring complex post-translational modifications, particularly glycosylation, which is essential for human-like protein functionality.

High Productivity and Flexibility
These cells exhibit fast proliferation rates and protein yields of 1–5 grams per liter of culture.
They are easily genetically modified, supporting both transient and stable expression studies.

Specialized CHO Cell Lines
CHO-K1 Cells
A widely used derivative of the original CHO cell line, CHO-K1 cells are optimized for producing therapeutic proteins and recombinant antibodies. Researchers can tailor CHO-K1 cells to enhance protein expression and achieve specific glycosylation profiles, making them indispensable in developing targeted biopharmaceutical therapies.

Applications in Biopharmaceuticals
CHO cells are at the forefront of therapeutic protein production, offering solutions to some of the most challenging protein expression problems. Key applications include:

Monoclonal Antibody Production: CHO cells are integral to producing antibodies with consistent quality and functionality.
Recombinant Protein Therapeutics: Their ability to mimic human protein modifications makes them a preferred choice for biotherapeutics.
Vaccine Development: CHO cells support safe and efficient production of vaccines, contributing to global health initiatives.

Conclusion
CHO cells have revolutionized the field of biotechnology, serving as a cornerstone for the production of recombinant proteins and monoclonal antibodies. Their unique combination of biosafety, scalability, and ability to mimic human post-translational modifications ensures their continued dominance in biopharmaceutical manufacturing. As biotechnology advances, CHO cells remain an invaluable resource for addressing the challenges of modern medicine and enabling the development of innovative therapies.