HT-29 Cells Catalogue number: 300215

HT-29 Cells: A Key Model for Colorectal Cancer Research

The HT-29 cell line, derived from a Grade II human colorectal adenocarcinoma, is an essential research tool in the study of human colon cancers. Isolated from a primary tumor in a 44-year-old female in 1964, HT-29 cells have been pivotal in enhancing our understanding of cancer cell adhesion and invasion mechanisms. These cells, exhibiting characteristics similar to mature intestinal cells, such as enterocytes, offer valuable insights into the dynamics of food digestion and nutrient bioavailability.

Key Characteristics of HT-29 Cells
HT-29 cells are commonly used to study the behavior of human colorectal cancer cells and closely resemble the biology of the human colon. Their ability to express differentiation pathways, particularly under specific conditions like glucose deprivation or exposure to inducers such as butyrate, makes them an invaluable model for exploring the molecular processes involved in cell differentiation and cancer progression. Additionally, HT-29 cells are sensitive to conventional colorectal cancer chemotherapies, such as 5-fluorouracil and oxaliplatin, enabling them to serve as an effective model for chemotherapy research.

Applications in Cancer Research and Therapy
HT-29 cells have also been utilized as a xenograft tumor model, where they provide a platform for in vivo studies that mimic the behavior of colon tumors in the human body. This application allows researchers to investigate tumor growth, metastasis, and the efficacy of therapeutic agents in more realistic, in vivo settings. The ability of HT-29 cells to closely mimic the biology of human colon cancer tumors makes them essential for testing novel cancer therapies and understanding the complexities of tumor development and metastasis.

Conclusion
In conclusion, HT-29 cells are a cornerstone in the study of colorectal cancer, offering critical insights into the molecular mechanisms of cancer cell differentiation, progression, and treatment. Their sensitivity to chemotherapy and utility in both in vitro and in vivo research models underscore their importance in advancing cancer research and therapeutic development. The HT-29 cell line continues to be a valuable resource in the fight against colorectal cancer.